To determine whether physciosporin affects the expression of cancer stemness markers in CRC cells, we monitored the protein expression level of aldehyde dehydrogenase-1 (ALDH1), cluster of differentiation 133 (CD133), CD44, Lgr5, and Musashi-1 (Msi-1) in CSC221 cells. This evidence concerns the gene PROM1 and colorectal carcinoma.