CASP3 and rheumatoid arthritis: In another study by Yan et al. in 2012, Andrographolide (8) (10, 20 and 30 μM) showed a highly anti-rheumatic activity on RA-FLS from RA patients cultured in vitro via inducing apoptosis, which was associated with decrease of the Bcl-2/Bax ratio, promotion of Cyt-C release, and activation of Caspase-3 [62].