S100A16, combining with S100A1‐S100A14 and S100A7, encodes in two tandem clusters on chromosome 1q21, which plays a powerful role in epidermal differentiation complex (EDC).33 As a novel detected S100 family member, S100A16 was observed over‐expressed in many malignancies.34 Nariaki et al demonstrated that S100A16 might increase the expression level of Oct4 and Nanog in cancer stem‐like cells in Yumoto human cervical carcinoma cells.35 According to our findings, S100A16 is highly expressed in bladder cancer, consistent with previous studies above. Here, S100A16 is linked to cancer.