ANO1 and neoplasm: ANO1 is located on chromosome 11q13, and amplification of 11q13 is a common event in cancers from multiple anatomical sites.25, 26ANO1 is upregulated and correlates with poor prognosis in several cancers.27, 28, 29 A previous study has also found that positive ANO1 is a promising biomarker to predict the unfavorable outcome for ESCC patients even in precancerous lesions.30 Our high‐throughput data showed that ANO1 expression was significantly upregulated in ESCC tumor tissues at both mRNA and protein levels.