Further, activity of brain and heart N-acylphosphatidylethanolamine phospholipase D (N-APE-PLD), the enzyme catalysing conversion of N-acylphosphatidylethanolamines to acyl ethanolamides, has been reported to increase ~15 folds from neonatal stage to adulthood in male Wistar rats while that of liver N-APE-PLD stayed unchanged with increasing age48, making it mechanistically unlikely that the that the reduced levels of multiple acyl ethanolamide species that we have observed in the T2D and T2D-DN plasma is due to their decline with age. This evidence concerns the gene GPLD1 and type 2 diabetes mellitus.