INS and atrial fibrillation: In the absence of insulin, BCNU/AF treatment for 1 h resulted in up-regulation of 214 and down-regulation of 799 phosphosites and 2 h treatment resulted in up-regulation of 509 and down-regulation of 1586 sites (absolute fold change > 1.5, adjusted p < 0.05 by moderated t-test, Fig. 2c, Supplementary Fig. 2d, e, Supplementary Data 7).