Fresh lung specimens fixed in formalin or stained in H&E are shown in Fig 1A–H The loss of CHUK/IKKα mRNA expression in the urethane-induced large lung adenomas (which solely developed in the Tmx-treated IKKαf/f:Rosa-fLacz:Sftpc-CreERT2 mice) was confirmed by qRT-PCR analysis of CHUK/IKKα exons 6 and 7, which are flanked by LoxP sites that are specifically deleted by Tmx-induced Sftpc-CreERT2–mediated recombination; and the lungs of the same mice as expected are X-gal positive (Fig S1). Here, TMX1 is linked to lung adenoma.