In order to confirm an effective inhibition of EZH2 by MC4040 and MC4041 in a cellular context, U-87, GL1 and HF were treated with DMSO (ctr), or with MC4040, or with MC4041 (both at 25 μM for 72 h), and the levels of H3K27me3 were analysed by western blot. This evidence concerns the gene EZH2 and hydrops fetalis.