The mucosa-associated microbiome was analyzed from the stapled colorectal “donut.” The results of this study showed that in the patient group without the C-seal, AL was significantly associated with a lower biodiversity of the microbiome and a higher abundance of the mucin-degrading microbiome families Lachnospiraceae and Bacteroidaceae compared with matched patients who did not develop AL. The gene discussed is MUC5AC; the disease is axial length measurement.