SP1 and metabolic syndrome: When wheat germ agglutinin (WGA) affinity captured O-GlcNAcylated proteins are stained via Western blot with YY1- and Sp1-specific antibodies (Fig 5C), lysates from MetS swine exhibit higher levels of O-GlcNAcylated YY1 and Sp1 variants compared to those of LD swine (Fig 5C, lanes B).