HCT116+3+5 cells are derived from MLH1- and MSH3-deficient parental HCT116 CRC cells where 1 copy of human chromosome 3 (correcting MLH1 deficiency) and 1 copy of human chromosome 5 (correcting MSH3 deficiency) have been transferred (41). The gene discussed is MSH3; the disease is colorectal carcinoma.