First, to define the time course of editing or indel introduction within 24 hr post treatment with SaCas9/UL30-5, we performed deep sequencing of the CRISPR target site from SaCas9/UL30-5-transduced and WT HSV-1-infected HFFs in the presence or absence of the viral DNA synthesis inhibitor, phosphonoacetic acid (PAA), at different time points post infection. Here, RPL7 is linked to infection.