PCK2 and cancer: Indeed, ISR genes including Trib3, Aldh1l2 and Pck2 are among the top transcripts induced by leucine starvation in HEK293 cells 71; moreover, Slc7a5 loss in human cancer cell lines has been shown to induce this stress response 19, 72, 73; we detect aberrant activity of the amino acid sensor mTORC1; and transcriptomic analysis did not reveal changes in other potentially linked pathways, dopamine and thyroid hormone systems.