In addition, similar increases in mRNA levels of stemness genes were detected in equivalent clones from other CRC cells of different malignancy (Ahmed et al., 2013), such as HT‐29 (Dukes C/D) (Fig. S2A) and SW‐480 (Dukes B) (Fig. S2B), which are positive and negative, respectively, for activating mutations in the PIK3CA gene, a common genetic trait found in CRC tumors. This evidence concerns the gene PIK3CA and colorectal carcinoma.