Afterwards, we performed co-immunoprecipitation assay and the data elucidated that LHX9 could interact with beta-catenin (Fig. 9), similarly, LHX4 has also been reported to bind to beta-catenin, which then translocated into cell nuclear and facilitated the association of TCF4 with LHX4/beta-catenin complex, and the complex could transactive downstream genes involved in the progression of human colorectal cancer [7]. This evidence concerns the gene TCF4 and colorectal cancer.