To see whether other LHX family members had similar expression changes, we further conducted RT-qPCR experiment to measure the relative expression of LHX1 to LHX9 (LHX1–9) with U2OS and hFOB1.19 cells, and the data showed that the expression of LHX 1, 7, 8 was lower, whereas the expression of LHX2, 4, 5, 6, 9 was higher in OS tumor tissues, furthermore, LHX3 expression had no significant change in our experiment (Additional file 1: Figure S1A). This evidence concerns the gene LHX1 and neoplasm.