In our western blotting analysis of the NF-κB pathway, NF-κB (p65) expression increased over time as the infection progressed in both TC0668wt- and TC0668mut-infected cells, but NF-κB (p65) levels in TC0668wt-infected cells were significantly higher than those in TC0668mut-infected cells at all time points (P < 0.05). The gene discussed is NFKB1; the disease is infection.