Prominent non-kidney off-target populations17, absent in adult human kidney (Fig. 1c, Supplementary Fig. 3B), were found in D29 organoids, including melanoma-like cells (PMEL), SOX2-positive(+) neuronal precursors, STMN2+ neuron-like cells, and MYOG+ muscle-like cells, as reported previously14–18. The gene discussed is SOX2; the disease is melanoma.