Furthermore, in parallel assays to confirm the anticancer role of garcinol, consistent with earlier results, we demonstrated that treatment with 2.5 μM or 5 μM garcinol, significantly suppressed the expression of N-cadherin, vimentin, and slug proteins, while conversely upregulating the expression of E-cadherin protein (Figure 2H), thus indicating that garcinol attenuates epithelial-to-mesenchymal transition (EMT) and the metastatic phenotype of GBM cells. The gene discussed is VIM; the disease is glioblastoma.