Sequel to our data demonstrating that garcinol suppresses the cancer stem cell-like phenotype of U87MG and GBM8401 cells in vitro, and that garcinol also suppresses the formation and growth of tumor in mice GBM models by upregulating hsa-miR-181d expression and inhibiting STAT3/5A activation, we further investigated if indeed these findings could be replicated in GBM primary culture cells. This evidence concerns the gene STAT3 and cancer.