TNFAIP3 and escherichia coli infection: To testify the possible regulation between miR-19b-3p and TNFAIP3 in the perspective of meningitic E. coli infection, the dual-luciferase reporter plasmid was constructed by cloning TNFAIP3 3’UTR binding region into psiCHECK-2 plasmid (Figure 3B).