Taken together, our data demonstrate the effect of Lanatoside C in modulating Wnt/β-catenin signaling, which could enhance the importance of inhibitors for β-catenin and its transcriptional targets including c-MYC, Cyclin D1, and CDK6 may serve as potential targets for developing novel therapeutic drugs against human cancers (Figure 6). This evidence concerns the gene CCND1 and cancer.