The expression levels of selected DEGs were validated by qRT‐PCR, and Wnt/β‐catenin involvement was validated by luciferase assays, western blotting and immunohistochemical analysis in vitro and in vivo. These results provide a comprehensive overview of the transcriptome in GSTZ1‐1‐overexpressing Huh7 cells and indicate that GSTZ1‐1 may play a tumour suppressor role by inactivating the Wnt/β‐catenin signalling pathway. Here, GSTZ1 is linked to neoplasm.