To recapitulate the recurrence of LCSCs following chemotherapy, A549 (wt P53), H460 (wt P53), and H661 (mt P53) human lung cancer cell lines were treated with cisplatin at concentrations ranging from 0.1 μM to 100 μM to determine the IC50 for the initial treatment, as previously reported [12]. This evidence concerns the gene TP53 and lung carcinoma.