Further evidence that IR-780 induced a more pronounced effector phenotype was attained by the assessment of multiple CTL effector markers through real-time qPCR of the whole tumor mRNA, demonstrating increased expression of IFN-γ, TNF-α, Granzyme B, EOMES, Perforin, BLIMP-1, FOXO3, and CXCL10 (Supplementary Figure 6). This evidence concerns the gene TNF and neoplasm.