For BRAF gene mutation in NSCLC detection, analysis was carried out by utilizing molecular methods including DNA extraction from FFPE biopsies [35], such methods can lead to depletion of tissue samples or the samples themselves are already not sufficient for such molecular approach because most of the biopsies that had been used in the diagnosis are bronchial or core needle transthoracic specimens. The gene discussed is BRAF; the disease is non-small cell lung carcinoma.