PTEN, as a negative regulator of PI3K/AKT signaling, was frequently observed being activated in myocardial ischemic injured tissues and reactivation of PI3K/Akt/GSK3β signaling by loss function of PTEN attenuated the myocardial ischemic injury [33] In the present study, we also observed that the expression of PTEN (both in mRNA and protein levels) was significantly upregulated in the renal tissues of Cis-induced AKI mice. Here, AKT1 is linked to acute kidney injury.