Both the leukocytospermia and varicocele groups showed significantly lower levels of ghrelin (P = 0.0001), obestatin (P = 0.0001 and P = 0.006, respectively), and GSH/GSSG ratio (P = 0.003 and P = 0.0001, respectively) compared to those measured in the control group. The gene discussed is GHRL; the disease is varicocele.