Consistent with these previous findings, in our study, rat tissues exhibited significantly enhanced expression and activity of TGF-β1, Smad2, and Smad3 after 7 days of treatment with EG, but TGF-β1 overexpression related to renal injury induced by hyperoxaluria was prevented by RSG injection in the rat model. This evidence concerns the gene TGFB1 and Hyperoxaluria.