FZD5 and infection: Small interfering RNA-mediated knock-down of WNT pathway components, including WNT5A, FZD5, FZD9, LRP6, CTNNB1, and DVL2 diminished intracellular bacterial burden over 1-2 days of infection, further supporting the notion that intracellular survival of E. chaffeensis in this cell line was facilitated by the action of endogenous WNT ligands (42).