PLCXD3 and Hyperglycemia: Finally, to investigate whether the reduced expression of PLCXD3 was due to the exposure of hyperglycemia status, expression of PLCXD3 was measured in INS-1 (832/13) cells cultured for a short term (24 h) at 11.1 mM (control) and 16.7 or 22.2 mM glucose at mRNA and protein levels.