In order to exam whether diabetes or Dapagliflozin affects the expression of bone related genes, we selected four osteocytes related genes to put through a q-PCR test: alkaline phosphatase (ALP), markers that indicate the mineralization ability of osteoblasts (OBs); Osterix (OSX), a specific transcription factor of OBs which indicates the generation rate of OBs; osteocalcin (OCN), a marker of bone turnover; and osteopontin (OPN), an indication of osteocytes proliferation. The gene discussed is SPP1; the disease is diabetes mellitus.