CD4 and neoplasm: To address the immune microenvironment, we analyzed the population of CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+), Natural killer cells (NK, CD3−NKp46+), regulatory T cells (Treg, CD4+Foxp3+), macrophages (CD11b+F4/80+), and myeloid-derived suppressor cells (MDSC, CD11b+Gr-1+) in spleen and tumor tissues by flow cytometry.