For bladder cancer cell T24, p-Akt inhibition effect was even faster, which began at 1 h post incubation and reached the lowest point at 24 h (Figure 8B), suggesting that CuC effectively inhibited the Akt signaling in prostate cancer, bladder cancer, and liver cancer cells, and the blocking of Akt phosphorylation at Ser473 was in a time-dependent manner. The gene discussed is AKT1; the disease is liver cancer.