We took the Wnt/beta-catenin signaling pathway as an example, and saw that the expression profiles of Wnt7a, APC, Wnt10a, and FZD8 showed similar patterns in the E45, E55, and E65 groups and E60, E120, and NB groups, while other genes showed different expression profiles in these two groups. The gene discussed is CTNNB1; the disease is neuroblastoma.