Given that PIM1 has been shown to induce drug resistance in other cancers by evading apoptosis33,42,43, expression of apoptotic regulators and known substrates downstream of PIM1 were investigated in SH-SY5Y and CHLA-20 cells, following gRNA-induced overexpression of PIM1. Specifically, expression of BAD44 and PRAS4045 was analysed. The gene discussed is PIM1; the disease is cancer.