VIM and melanoma: Figure 2B shows the results obtained from the preliminary analysis of Matrigel invasion assay. When melanoma cells were stimulated with BAI, there was an obvious decline in the relative invasive rate compared to the control group (P<0.001). It is well known that the activation of angiogenesis depends on MMP-2 and vimentin, which are known to participate in the epithelial-mesenchymal transition (21,22). As shown in Figure 2C, MMP-2 and vimentin expression was inhibited due to the treatment of BAI compared with the control (P<0.001).