We then assessed the effect exerted by BBI608 and paclitaxel on constitutive nuclear expression of pStat3 in EOC tumour tissues through immunohistochemical analysis and found that compared with the vehicle control, BBI608, either independently or jointly with paclitaxel, inhibited Stat3 activation in a moderate manner by inhibiting phosphorylation at Tyr705 (Figure 5D,F). This evidence concerns the gene STAT3 and neoplasm.