Western blot assay revealed that the expression of SIRT1, PGC-1α, NRF1 and TFAM proteins of OBH-HF was lower than those of WKY-ND (P < 0.01) while HQQR treatment for 10 weeks could up-regulate their protein levels (P < 0.05) (Figure 4C,D). The gene discussed is NRF1; the disease is hydrops fetalis.