Cells were transfected with multiplicity of infection (MOI) (shMTHFD2‐L) and high MOI (shMTHFD2‐H), and shMTHFD2‐H significantly suppressed the protein expression of MTHFD2 when compared with shMTHFD2‐L in both A549 and H1299 (Figure 3C). This evidence concerns the gene MTHFD2 and infection.