According to the literature search and functional prediction of these significantly up‐regulated DEGs, 20 highly abundant candidates in clinical NSCLC samples were chosen for further screening in H1299 cells using qRT‐PCR, and up‐regulated MTHFD2 which is a mitochondrial enzyme was ultimately selected for subsequent investigations (Figure 1E). Here, MTHFD2 is linked to non-small cell lung carcinoma.