Given such important roles in the cancer cells proliferation, MTHFD2 has been recently considered to be a promising target for multiple types of cancer.21, 22, 23 During the treatments of acute myeloid leukaemia and colorectal cancer, targeting MTHFD2 can markedly suppress the tumour progression both in vitro and in vivo.16, 21 Importantly, our findings here extend the therapeutic function of MTHFD2 to NSCLC, which targeting MTHFD2 can be a potentially valuable approach in the clinic. Here, MTHFD2 is linked to cancer.