An assorted methylation detection of HOXA9, ONECUT2, PCDH17, PENK, TWIST1, VIM and ZNF154 from urinary sample displayed great prediction accuracy of bladder cancer from clinical hematuria patients, which means unnecessary invasive test could be avoided for cancer patients at early stage and during the follow-up after operation. The gene discussed is TWIST1; the disease is urinary bladder carcinoma.