To explore the functional significance of VCAN-V1 upregulation on cell migration and invasion properties in NPM1-mutated leukemia, OCI-AML3 cells were stably infected with shRNA lentivirus targeting VCAN and the decreased levels of VCAN-V1 mRNA and protein were successfully confirmed (Figure 4A and 4B). This evidence concerns the gene NPM1 and leukemia.