Knockdown of HPS1 (HPS1KDi6e7, HPS1KD ATG) and -5 (HPS5KD e12i12, HPS5KD ATG) results in a severe renal phenotype with pericardial effusion, yolk sac edema and protein loss, while HPS3 (HPS3KDe7i7, HPS3KD ATG) and -4 (HPS4KD e8i8, HPS4 KD ATG) induces, if present at all, only mild pericardial effusion, yolk sac edema and no protein loss in zebrafish larvae. Here, HPS4 is linked to pericardial effusion.