We focused on the roles of KHSRP in lung cancer metastasis in vitro and in vivo, identified the protein molecules that interacted with KHSRP by using Coimmunoprecipitation (Co-IP) and proteomic analyses, verified the functions and effects of the interacting proteins in promoting metastasis, elucidated the molecular mechanism of metastasis, demonstrated the potential tumor metastasis-related signaling pathway, and validated its clinical significance in a mass of lung cancer specimens. Here, KHSRP is linked to lung cancer.