The large-sample pooled analysis of nine homogeneous case-control studies demonstrated an association between MTHFR C677T variant and increased risk of ischemic stroke as follows: a 23% (95% CI 6–43%) higher risk of ischemic stroke was observed in the MTHFR C677T mutation group than in the control group with no mutation, and up to 32% (95% CI 9–59%) higher risk was observed specifically in the Chinese subgroup. This evidence concerns the gene MTHFR and ischemic stroke.