Accumulating evidence shows that ischaemic injury and inflammation account for the pathogenic progression of stroke.18, 27 In the present study, we found that the level of the macrophage marker, CD68, and T cell marker, CD3, expression were lower in the MCAO + TIM‐4 mAb group compared with that in the MCAO group, indicating that TIM‐4 mAb reduced macrophage and T cell infiltration. This evidence concerns the gene TIMD4 and Stroke.