To further decipher the molecular mechanism by which knocking down LINC00467 expression suppressed the proliferation and invasion of CRC cells in vitro, we assessed the mRNA and protein level of the proliferated markers Cyclin D1, Cyclin A1, CDK2 and CDK4, as well as, the epithelial marker E‐cadherin, and the mesenchymal markers Twist1 in HT29 cell lines, using qRT-PCR and western blotting techniques. This evidence concerns the gene CDK4 and colorectal carcinoma.