Moreover, by analysing the GSE37364 datasets, we found that cyclin D1, CDK4, and Twist1 were significantly up-regulated in CRC tissues, whereas E-cadherin was significantly down-regulated in CRC tissues when compared with the non-tumour tissues (all p < 0.05, Supplemental Fig. 1). The gene discussed is TWIST1; the disease is colorectal carcinoma.