Quantitation of FOLR1 transcript by pairwise comparisons revealed that in comparison to BNFN, condition of B12 deficiency in combination with folate deficiency (BDFD) and folate normal (BDFN) resulted in an increased transcript in all the tissues whereas in combination with folate over-supplementation (BDFO) it was increased in the brain and kidney (BNFN vs BDFN, BDFD, BDFO). The gene discussed is FOLR1; the disease is folate deficiency.