To mirror-image the loss of DAPK1 protein observed at tumor invasion front of colorectal tumors, we first established CRISPR/Cas9-driven DAPK1 ko HCT116 cell lines using two different single guide RNAs (sgRNA1 and 2) (Supplementary Fig. 1, a, b). This evidence concerns the gene DAPK1 and colorectal neoplasm.