TFAP2C and breast cancer: In addition, increased levels of TFAP2A expression remained statistically significant even after removing HT1197 cells from the analysis (p = 0.0238; Mann–Whitney U test).This was additionally confirmed by western blotting showing that while expression of both PPARɣ isoforms (ɣ1 and ɣ2) was variable across BC cell lines, the basal-squamous cell lines SCaBER, 5637, HT1376, and HT1197 expressed relatively high TFAP2A and TFAP2C expression (Fig. 4d) in comparison to luminal and “non-type” lines.