RNA-seq examination of the mutated cancers confirmed the presence of aberrant splicing of NOTCH1, where the final exon was spliced into a newly formed splice acceptor site in the 3′ UTR of NOTCH1. As a result of this, 158 coding bases were deleted, including those encoding the negative regulatory PEST domain of the mature NOTCH1 protein. Here, NOTCH1 is linked to cancer.