In this study, we characterized TCR and BCR repertoires in both pediatric and adult AML by detecting and analyzing the CDR3 sequences in TCR α, β, γ, and δ chains and B cell immunoglobulin (Ig) heavy (IgH) and light (IgL, IgK) chains from the RNA-seq data in AML patients and non-tumor donors. Here, BCR is linked to acute myeloid leukemia.